Characterization and DFT Studies for Green Synthesis of Silver Nanoparticles by Morphine Ampules and their Anti-proliferation Activity

Document Type : Research Paper


1 Department of Nanotechnology, Mineral Industries Research Center (MIRC), Shahid Bahonar University of Kerman, Kerman, Iran.

2 Department of Materials Science and Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman, Iran.

3 Cellular and Molecular Research Center, Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.


This study is a green approach for the synthesis of silver nanoparticles (AgNPs) using morphine ampoules (MO) as reducing and capping agents. The toxicity effects of prepared particles were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on breast cancer cells. UV–visible spectrum, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), environmental scanning electron microscopy (ESEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS) techniques were employed for characterization of prepared particles. A review mechanism has been done based on density functional theory (DFT). The results confirmed the formation of spherical and crystalline AgNPs with the average particle size of 50nm. Also, DFT analysis revealed that the reduction was occurred by connection of MO constituents to the NO3 part of AgNO3. In addition of reducing effect, the capping characteristics of MO biomolecules were proved by FTIR spectra. Moreover, comparison of Anti-proliferation Activity of MO-AgNPs was 69% higher than MO at similar dosage. Therefore, in addition to use MO as a painkiller in the treatment of cancer, it can also be used as a factor for the synthesis of AgNPs with enhanced its anticancer properties.