Fabrication of Amniotic Membrane coated Silk Fibroin nanofibers Containing Thymol for Antibacterial Wound Dressing

Document Type : Research Paper

Authors

1 Biomaterials Lab, School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran.

2 Biomaterials Lab, School of Metallurgy and Materials Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran, Iran

10.22059/jufgnsm.2025.01.01

Abstract

Bacterial wound infection delays wound healing and may lead to life-threatening complications. Hence wound dressing materials are not restricted to stop blood loss, but also to protect the wound from bacterial infection and to accelerate the wound healing process. In this study, biopolymeric nanofibers of Silk Fibroin (SF) and decellularized human Amniotic membrane (dehAM) were fabricated by electrospinning technique to be used in wound healing. Thymol, a natural antibacterial substance, solutions containing 5% wt/wt, 10%wt/wt and 15% wt/wt thymol was added to the SF nanofibers to increase the sustained release. Scanning Electron Microscopy and Attenuated Total Reflectance Fourier-Transform Infrared (FTIR-ATR) were used to examine the nanofibers' morphology and chemical characteristics. Water contact angle and water vapor transmission rate of dressings were measured. When the thymol concentration of the dressing was increased, the wettability of the dressing decreased by an average of 54%. The dressings' antimicrobial activity was determined using the colony count method, which dressings effectively inhibited the development of E. coli and S. aureus. Furthermore, cell attachment, cell viability, and in vitro cytotoxicity experiments have demonstrated the biocompatibility and cytocompatibility of the dressings, as well as their suitability for MSCs attachment. Altogether, the produced dressing was shown to have immense potential as a wound dressing.

Keywords

References

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Journal of Ultrafine Grained and Nanostructured Materials
Volume 58, Issue 1
June 2025
Pages 1-14

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History

  • Receive Date: 16 October 2024
  • Revise Date: 23 April 2025
  • Accept Date: 24 April 2025

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