PHEMA- PANI coating doped with silver nanoparticles for prevention of catheter-associated urinary tract biofilm infections

Document Type : Research Paper

Authors

1 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Dental and Periodontal Research Center, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

3 School of Engineering, Damghan University, Damghan, Iran

4 Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

10.22059/jufgnsm.2024.02.08

Abstract

Long-term indwelling urinary catheters are associated with complications like infection and encrustation, which have brought patients burdens of health problems. Considering the damages caused by urinary tract infections, development of antibiofilm catheter coatings is a practical way to address this issue. Herein, we developed a PHEMA (poly(2- hydroxyethyl methacrylate))-PANI (polyaniline) based coating for stabilizing silver nanoparticles resulting in a high-performance antibiofilm catheter. For this purpose, silicone catheters were functionalized with OH groups and then 2- hydroxyethyl methacrylate (HEMA) was polymerized on the catheter by atom transfer radical polymerization (ATRP). The OH groups of PHEMA were converted into amine groups by reaction with para-anthranilic acid, and in the next step, PANI was produced by oxidation-reduction polymerization. In order to investigate the synergistic effects of silver nanoparticles on the antibacterial property of polyaniline, Ag nanoparticles were coated on polyaniline. Coated catheters were evaluated at each step using attenuated total reflection-fourier transform infrared (ATR-FTIR), scanning electron microscope (SEM), thermal gravimetric analysis (TGA), and atomic force microscopy (AFM). The water contact angle and consequently the hydrophilicity of the coated catheter have increased from 121˚ for the uncoated catheter to 101˚ for catheter-PHEMA-PANI and 73˚ for the catheter-PHEMA-PANI-Ag. Therefore, a hydrophilic PHEMA-PANI-Ag-coated catheter was developed with excellent thermal stability, antibacterial and antibiofilm properties against Escherichia coli and Pseudomonas aeruginosa during 24 and 48 hours and also improved biocompatibility on L929 fibroblast cells. It is concluded that the PHEMA-PANI-Ag-coated catheter with significant activity against antibiofilm formation is a potential candidate for indwelling urinary catheters and supports further clinical investigations.

Keywords

References

  1. Hassan M, Brede DA, Diep DB, Nes IF, Lotfipour F, Hojabri Z. Efficient inactivation of multi-antibiotics resistant nosocomial enterococci by purified hiracin bacteriocin. Advanced pharmaceutical bulletin. 2015;5(3):393.
  2. Organization WH. Antimicrobial resistance: global report on surveillance: World Health Organization; 2014.
  3. Shams F, Hasani A, Rezaee MA, Nahaie MR, Hasani A, Haghi MHSB, et al. Carriage of class 1 and 2 integrons in quinolone, extended-spectrum-β-lactamase-producing and multi drug resistant E. coli and K. pneumoniae: High burden of antibiotic resistance. Advanced pharmaceutical bulletin. 2015;5(3):335.
  4. Sharifi Y, Hasani A, Ghotaslou R, Naghili B, Aghazadeh M, Milani M, et al. Virulence and antimicrobial resistance in enterococci isolated from urinary tract infections. Advanced pharmaceutical bulletin. 2013;3(1):197.
  5. Percival SL, Suleman L, Vuotto C, Donelli G. Healthcare-associated infections, medical devices and biofilms: risk, tolerance and control. Journal of medical microbiology. 2015;64(4):323-34.
  6. Weinstein RA, Darouiche RO. Device-associated infections: a macroproblem that starts with microadherence. Clinical infectious diseases. 2001;33(9):1567-72.
  7. Dadi NCT, Radochová B, Vargová J, Bujdáková H. Impact of healthcare-associated infections connected to medical devices—an Update. Microorganisms. 2021;9(11):2332.
  8. N. Hoiby, O. Ciofu, H.K. Johansen, Z.J. Song, C. Moser, P.O. Jensen, S. Molin, M. Givskov, T. Tolker-Nielsen, T. Bjarnsholt, The clinical impact of bacterial biofilms, Int. J. Oral Sci. 3 (2011) 55–65.
  9. W. Hu, L. Li, S. Sharma, J. Wang, I. McHardy, R. Lux, Z. Yang, X. He, J.K. Gimzewski, Y. Li, W. Shi, DNA builds and strengthens the extracellular matrix in Myxococcus xanthus biofilms by interacting with exopolysaccharides, PLoS One 7 (2012) e51905.
  10. B. Lee, C.K. Schjerling, N. Kirkby, N. Hoffmann, R. Borup, S. Molin, N. Hoiby, O. Ciofu, Mucoid Pseudomonas aeruginosa isolates maintain the biofilm formation capacity and the gene expression profiles during the chronic lung infection of CF patients, APMIS 119 (2011) 263–274.
  11. P.O. Jensen, M. Givskov, T. Bjarnsholt, C. Moser, The immune system vs. Pseudomonas aeruginosa biofilms, FEMS Immunol. Med. Microbiol. 59 (2010) 292–305.
  12. Hernández-Jiménez, Enrique, Rosa Del Campo, Victor Toledano, Maria Teresa Vallejo-Cremades, Aurora Muñoz, Carlota Largo, Francisco Arnalich, Francisco García-Rio, Carolina Cubillos-Zapata, and Eduardo López-Collazo. "Biofilm vs. planktonic bacterial mode of growth: which do human macrophages prefer?." Biochemical and biophysical research communications441, no. 4 (2013): 947-952.
  13. Nicolle LE. Catheter associated urinary tract infections. Antimicrobial resistance and infection control. 2014;3:1-8.
  14. Sabbuba N, Mahenthiralingam E, Stickler DJ. Molecular epidemiology of Proteus mirabilis infections of the catheterized urinary tract. Journal of clinical microbiology. 2003;41(11):4961-5.
  15. Warren JW. Catheter-associated urinary tract infections. International journal of antimicrobial agents. 2001;17(4):299-303.
  16. Sarvari R, Naghili B, Agbolaghi S, Abbaspoor S, Bannazadeh Baghi H, Poortahmasebi V, et al. Organic/polymeric antibiofilm coatings for surface modification of medical devices. International Journal of Polymeric Materials and Polymeric Biomaterials. 2022:1-42.
  17. Khalil-Allafi J, Daneshvar H, Safavi MS, Khalili V. A survey on crystallization kinetic behavior of direct current magnetron sputter deposited NiTi thin films. Physica B: Condensed Matter. 2021 Aug 15;615:413086.
  18. Mozetič M. Surface modification to improve properties of materials. Materials. 2019 Jan 31;12(3):441.
  19. Safavi MS, Khalil-Allafi J, Ahadzadeh I, Walsh FC, Visai L. Improved corrosion protection of a NiTi implant by an electrodeposited HAp-Nb2O5 composite layer. Surface and Coatings Technology. 2023 Oct 15;470:129822.

 

 

  1. Fadeeva E, Truong VK, Stiesch M, Chichkov BN, Crawford RJ, Wang J, et al. Bacterial retention on superhydrophobic titanium surfaces fabricated by femtosecond laser ablation. Langmuir. 2011;27(6):3012-9.
  2. Dong Y, Li X, Bell T, Sammons R, Dong H. Surface microstructure and antibacterial property of an active-screen plasma alloyed austenitic stainless steel surface with Cu and N. Biomedical Materials. 2010;5(5):054105.
  3. Hickok NJ, Shapiro IM. Immobilized antibiotics to prevent orthopaedic implant infections. Advanced drug delivery reviews. 2012;64(12):1165-76.
  4. Wang X, Venkatraman SS, Boey FY, Loo JS, Tan LP. Controlled release of sirolimus from a multilayered PLGA stent matrix. Biomaterials. 2006;27(32):5588-95.
  5. Xu Q, Czernuszka JT. Controlled release of amoxicillin from hydroxyapatite-coated poly (lactic-co-glycolic acid) microspheres. Journal of Controlled Release. 2008;127(2):146-53.
  6. Andersen MJ, Flores-Mireles AL. Urinary catheter coating modifications: the race against catheter-associated infections. Coatings. 2019;10(1):23.
  7. Dai S, Gao Y, Duan L. Recent advances in hydrogel coatings for urinary catheters. Journal of Applied Polymer Science. 2023;140(14):e53701.
  8. Yu K, Alzahrani A, Khoddami S, Ferreira D, Scotland KB, Cheng JT, et al. Self‐Limiting Mussel Inspired Thin Antifouling Coating with Broad‐Spectrum Resistance to Biofilm Formation to Prevent Catheter‐Associated Infection in Mouse and Porcine Models. Advanced Healthcare Materials. 2021;10(6):2001573.
  9. Wang L, Zhang S, Keatch R, Corner G, Nabi G, Murdoch S, et al. In-vitro antibacterial and anti-encrustation performance of silver-polytetrafluoroethylene nanocomposite coated urinary catheters. Journal of Hospital Infection. 2019;103(1):55-63.
  10. Peng W, Liu P, Zhang X, Peng J, Gu Y, Dong X, et al. Multi-functional zwitterionic coating for silicone-based biomedical devices. Chemical Engineering Journal. 2020;398:125663.
  11. Stirpe M, Brugnoli B, Donelli G, Francolini I, Vuotto C. Poloxamer 338 affects cell adhesion and biofilm formation in escherichia coli: Potential applications in the management of catheter-associated urinary tract infections. Pathogens. 2020;9(11):885.
  12. McCoy CP, Irwin NJ, Donnelly L, Jones DS, Hardy JG, Carson L. Anti-adherent biomaterials for prevention of catheter biofouling. International journal of pharmaceutics. 2018;535(1-2):420-7.
  13. Roe D, Karandikar B, Bonn-Savage N, Gibbins B, Roullet J-B. Antimicrobial surface functionalization of plastic catheters by silver nanoparticles. Journal of antimicrobial chemotherapy. 2008;61(4):869-76.
  14. Ballo MK, Rtimi S, Pulgarin C, Hopf N, Berthet A, Kiwi J, et al. In vitro and in vivo effectiveness of an innovative silver-copper nanoparticle coating of catheters to prevent methicillin-resistant Staphylococcus aureus infection. Antimicrobial agents and chemotherapy. 2016;60(9):5349-56.
  15. Chatzinikolaou I, Finkel K, Hanna H, Boktour M, Foringer J, Ho T, et al. Antibiotic-coated hemodialysis catheters for the prevention of vascular catheter–related infections: a prospective, randomized study. The American journal of medicine. 2003;115(5):352-7.
  16. Li X, Li P, Saravanan R, Basu A, Mishra B, Lim SH, et al. Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties. Acta biomaterialia. 2014;10(1):258-66.
  17. Lehman SM, Donlan RM. Bacteriophage-mediated control of a two-species biofilm formed by microorganisms causing catheter-associated urinary tract infections in an in vitro urinary catheter model. Antimicrobial agents and chemotherapy. 2015;59(2):1127-37.
  18. Nowatzki PJ, Koepsel RR, Stoodley P, Min K, Harper A, Murata H, et al. Salicylic acid-releasing polyurethane acrylate polymers as anti-biofilm urological catheter coatings. Acta Biomaterialia. 2012;8(5):1869-80.
  19. Wang R, Neoh KG, Kang ET, Tambyah PA, Chiong E. Antifouling coating with controllable and sustained silver release for long‐term inhibition of infection and encrustation in urinary catheters. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2015;103(3):519-28.
  20. Gayani B, Dilhari A, Kottegoda N, Ratnaweera DR, Weerasekera MM. Reduced crystalline biofilm formation on superhydrophobic silicone urinary catheter materials. ACS omega. 2021;6(17):11488-96.
  21. Gu G, Erişen DE, Yang K, Zhang B, Shen M, Zou J, et al. Antibacterial and anti‐inflammatory activities of chitosan/copper complex coating on medical catheters: In vitro and in vivo. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2022;110(8):1899-910.

41 Wang, Shuyang, Zhengjin Wang, Yan Yang, and Tongqing Lu. "Swell induced stress in a hydrogel coating." Acta Mechanica Sinica 37, no. 5 (2021): 797-802.

  1. Dai, Simin, Yang Gao, and Lijie Duan. "Recent advances in hydrogel coatings for urinary catheters." Journal of Applied Polymer Science140, no. 14 (2023): e53701.
  2. Namsheer K, Rout CS. Conducting polymers: A comprehensive review on recent advances in synthesis, properties and applications. RSC advances. 2021;11(10):5659-97.
  3. Sarvari R, Agbolaghi S, Beygi-Khosrowshahi Y, Massoumi B. Towards skin tissue engineering using poly (2-hydroxy ethyl methacrylate)-co-poly (N-isopropylacrylamide)-co-poly (ε-caprolactone) hydrophilic terpolymers. International Journal of Polymeric Materials and Polymeric Biomaterials. 2019;68(12):691-700.
  4. Hatamzadeh M, Sarvari R, Massoumi B, Agbolaghi S, Samadian F. Liver tissue engineering via hyperbranched polypyrrole scaffolds. International Journal of Polymeric Materials and Polymeric Biomaterials. 2020;69(17):1112-22.
  5. Sarvari R, Massoumi B, Zareh A, Beygi-Khosrowshahi Y, Agbolaghi S. Porous conductive and biocompatible scaffolds on the basis of polycaprolactone and polythiophene for scaffolding. Polymer Bulletin. 2020;77:1829-46.
  6. Agbolaghi S, Abbaspoor S, Massoumi B, Sarvari R, Sattari S, Aghapour S, et al. Conversion of Face‐On Orientation to Edge‐On/Flat‐On in Induced‐Crystallization of Poly (3‐hexylthiophene) via Functionalization/Grafting of Reduced Graphene Oxide with Thiophene Adducts. Macromolecular Chemistry and Physics. 2018;219(4):1700484.
  7. Zhang H, Zhong H, Dou F, Wang C, Wang S. Electrospinning bifunctional polyphenylene-vinylene/heated graphene oxide composite nanofibers with luminescent-electrical performance. Thin Solid Films. 2021;725:138636.
  8. Shi N, Guo X, Jing H, Gong J, Sun C, Yang K. Antibacterial effect of the conducting polyaniline. 2006.
  9. Gallarato LA, Mulko LE, Dardanelli MS, Barbero CA, Acevedo DF, Yslas EI. Synergistic effect of polyaniline coverage and surface microstructure on the inhibition of Pseudomonas aeruginosa biofilm formation. Colloids and Surfaces B: Biointerfaces. 2017;150:1-7.
  10. Seshadri DT, Bhat NV. Use of polyaniline as an antimicrobial agent in textiles. 2005.
  11. Keyhanvar N, Zarghami N, Seifalian A, Keyhanvar P, Sarvari R, Salehi R, et al. The Combined Thermoresponsive Cell-Imprinted Substrate, Induced Differentiation, and" KLC Sheet" Formation. Advanced Pharmaceutical Bulletin. 2022;12(2):356.
  12. Sarvari R, Massoumi B, Jaymand M, Beygi-Khosrowshahi Y, Abdollahi M. Novel three-dimensional, conducting, biocompatible, porous, and elastic polyaniline-based scaffolds for regenerative therapies. RSC advances. 2016;6(23):19437-51.
  13. Badica P, Batalu N, Burdusel M, Grigoroscuta M, Aldica G, Enculescu M, et al. Antibacterial composite coatings of MgB2 powders embedded in PVP matrix. Scientific reports. 2021;11(1):9591.
  14. Akhlaghi-Ardekani, N., Mohebbi-Kalhori, D. and Karazhyan, R., 2021. Improving Antibacterial and Anti-biofilmic properties of urological catheters with Eucalyptus, Rosemary, Green tea and Ziziphora extracts by impregnation method.

 

  1. Massoumi, B., Sarvari, R. and Fakhri, E., 2024. Polyzwitterion coating based on PDMAEMA-block-PAAc for catheters with antibiofilm activities. International Journal of Polymeric Materials and Polymeric Biomaterials, pp.1-8.
  2. Massoumi, B., Sarvari, R., Fakhri, E. and Vojoudi Fakhrnezhad, M., 2024. Silicon surfaces coated with polydopamine and poly (2-hydroxyethyl methacrylate) for medical device applications. Polymer Bulletin, pp.1-15.
  3. Lam SJ, O'Brien-Simpson NM, Pantarat N, Sulistio A, Wong EH, Chen Y-Y, et al. Combating multidrug-resistant Gram-negative bacteria with structurally nanoengineered antimicrobial peptide polymers. Nature microbiology. 2016;1(11):1-11.
  4. Lewis K. Platforms for antibiotic discovery. Nature reviews Drug discovery. 2013;12(5):371-87.
  5. Pham P, Oliver S, Wong EHH, Boyer C. Effect of hydrophilic groups on the bioactivity of antimicrobial polymers. Polymer Chemistry. 2021;12(39):5689-703.
  6. Gizdavic-Nikolaidis MR, Bennett JR, Swift S, Easteal AJ, Ambrose M. Broad spectrum antimicrobial activity of functionalized polyanilines. Acta biomaterialia. 2011;7(12):4204-9.
Journal of Ultrafine Grained and Nanostructured Materials
Volume 57, Issue 2
December 2024
Pages 177-189

Files

History

  • Receive Date: 05 October 2023
  • Revise Date: 14 November 2024
  • Accept Date: 20 November 2024

Share

How to cite

Statistics