Characterization and DFT Studies for Green Synthesis of Silver Nanoparticles by Morphine Ampules and their Anti-proliferation Activity

Shafaei, Amineh; Khayati, Gholam Reza; Hoshyar, Reyhane

doi:10.22059/jufgnsm.2020.01.11

Characterization and DFT Studies for Green Synthesis of Silver Nanoparticles by Morphine Ampules and their Anti-proliferation Activity

Document Type : Research Paper

Authors

Amineh Shafaei ¹

Gholam Reza Khayati ²

Reyhane Hoshyar ³

¹ Department of Nanotechnology, Mineral Industries Research Center (MIRC), Shahid Bahonar University of Kerman, Kerman, Iran.

² Department of Materials Science and Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman, Iran.

³ Cellular and Molecular Research Center, Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.

10.22059/jufgnsm.2020.01.11

Abstract

This study is a green approach for the synthesis of silver nanoparticles (AgNPs) using morphine ampoules (MO) as reducing and capping agents. The toxicity effects of prepared particles were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on breast cancer cells. UV–visible spectrum, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), environmental scanning electron microscopy (ESEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS) techniques were employed for characterization of prepared particles. A review mechanism has been done based on density functional theory (DFT). The results confirmed the formation of spherical and crystalline AgNPs with the average particle size of 50nm. Also, DFT analysis revealed that the reduction was occurred by connection of MO constituents to the NO₃ part of AgNO₃. In addition of reducing effect, the capping characteristics of MO biomolecules were proved by FTIR spectra. Moreover, comparison of Anti-proliferation Activity of MO-AgNPs was 69% higher than MO at similar dosage. Therefore, in addition to use MO as a painkiller in the treatment of cancer, it can also be used as a factor for the synthesis of AgNPs with enhanced its anticancer properties.

Keywords

Green synthesis

Morphine ampoules

Breast cancer cells

Density functional theory

Biomaterials

Nanoparticles

1. Ramar M, Manikandan B, Marimuthu PN, Raman T, Mahalingam A, Subramanian P, et al. Synthesis of silver nanoparticles using Solanum trilobatum fruits extract and its antibacterial, cytotoxic activity against human breast cancer cell line MCF 7. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2015;140:223-8.

2. Al-Bahrani R, Raman J, Lakshmanan H, Hassan AA, Sabaratnam V. Green synthesis of silver nanoparticles using tree oyster mushroom Pleurotus ostreatus and its inhibitory activity against pathogenic bacteria. Materials Letters. 2017;186:21-5.

3. Elemike EE, Onwudiwe DC, Nundkumar N, Singh M, Iyekowa O. Green synthesis of Ag, Au and Ag-Au bimetallic nanoparticles using Stigmaphyllon ovatum leaf extract and their in vitro anticancer potential. Materials Letters. 2019;243:148-52.

4. Srinithya B, Kumar VV, Vadivel V, Pemaiah B, Anthony SP, Muthuraman MS. Synthesis of biofunctionalized AgNPs using medicinally important Sida cordifolia leaf extract for enhanced antioxidant and anticancer activities. Materials Letters. 2016;170:101-4.

5. Azizi Z, Pourseyedi S, Khatami M, Mohammadi H. Stachys lavandulifolia and Lathyrus sp. Mediated for Green Synthesis of Silver Nanoparticles and Evaluation Its Antifungal Activity Against Dothiorella sarmentorum. Journal of Cluster Science. 2016;27(5):1613-28.